利用条件性诱变研究大脑。

Using conditional mutagenesis to study the brain.

作者信息

Morozov Alexei, Kellendonk Christoph, Simpson Eleanor, Tronche Francois

机构信息

Unit on Behavioral Genetics, Laboratory of Molecular Pathophysiology, Department of Health and Humans Services (AM), National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892, USA.

出版信息

Biol Psychiatry. 2003 Dec 1;54(11):1125-33. doi: 10.1016/s0006-3223(03)00467-0.

DOI:10.1016/s0006-3223(03)00467-0

PMID:14643079

Abstract

Conditional genetic modifications are used to determine how individual molecules contribute to the function of defined neuronal circuits in the mouse brain. Among various techniques for these genetic modifications, the tetracycline transactivator and the Cre-loxP systems have proved to be most successful in recent years. Here we describe the basic principles, recent developments, and potential applications of these methodologies. We discuss their impact on the study of general brain function and their use for modeling different brain disorders.

摘要

条件性基因修饰用于确定单个分子如何对小鼠大脑中特定神经回路的功能产生影响。在这些基因修饰的各种技术中，四环素反式激活因子和Cre-loxP系统近年来已被证明是最成功的。在此，我们描述这些方法的基本原理、最新进展和潜在应用。我们讨论它们对一般脑功能研究的影响以及它们在不同脑部疾病建模中的应用。