Yáñez Leticia, Borja-Aburto Víctor H, Rojas Emilio, de la Fuente Hortensia, González-Amaro Roberto, Gómez Humberto, Jongitud Alejandro A, Díaz-Barriga Fernando
Laboratorio de Toxicologia, Ambiental Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano, Carranza No. 2405, Col. Lomas los Filtros, CP 78210, San Luis Potosí, Mexico
Environ Res. 2004 Jan;94(1):18-24. doi: 10.1016/s0013-9351(03)00047-1.
In this study, DDT-induced DNA damage on blood cells was analyzed both in vitro and in vivo. Peripheral blood mononuclear cells (PBMC) were isolated from healthy donors and incubated in the presence of three different concentrations (40, 80, and 100 microg/mL) of p,p'-DDT, p,p'-DDE, and p,p'-DDD at three different treatment times (24, 48, and 72 h). Then, DNA damage was assessed by the single-cell electrophoresis assay (comet assay) as well as by flow cytometry detection of hypodiploid cells (DNA content assay). All compounds induced significant DNA damage as shown by the comet assay. Accordingly, cells exposed to DDT, DDE, and DDD showed a significant increase in the percentage of hypodiploid cells compared with untreated PBMC. In agreement with the in vitro data, a significant correlation between blood levels of DDT, DDD, and DDE and DNA damage (comet assay) was found in women with different amounts of environmental exposure. This association remained significant after controlling for nutritional status, smoking habits, alcohol ingestion, and reported exposure to other pesticides. Although the precise biological importance remains to be explained, our results strongly suggest that DDT and its metabolites are able to induce DNA damage in PBMC both in vitro and in vivo.
在本研究中，对滴滴涕诱导的血细胞DNA损伤进行了体外和体内分析。从健康供体中分离出外周血单核细胞（PBMC），并在三种不同浓度（40、80和100微克/毫升）的p,p'-滴滴涕、p,p'-滴滴伊和p,p'-滴滴滴存在下，于三个不同处理时间（24、48和72小时）进行孵育。然后，通过单细胞电泳分析（彗星试验）以及流式细胞术检测亚二倍体细胞（DNA含量测定）来评估DNA损伤。彗星试验显示，所有化合物均诱导了显著的DNA损伤。因此，与未处理的PBMC相比，暴露于滴滴涕、滴滴伊和滴滴滴的细胞中亚二倍体细胞的百分比显著增加。与体外数据一致，在不同环境暴露量的女性中，发现滴滴涕、滴滴滴和滴滴伊的血液水平与DNA损伤（彗星试验）之间存在显著相关性。在控制营养状况、吸烟习惯、酒精摄入以及报告的其他农药暴露情况后，这种关联仍然显著。尽管确切的生物学重要性仍有待解释，但我们的结果强烈表明，滴滴涕及其代谢产物在体外和体内均能诱导PBMC中的DNA损伤。
