Qian Lichuan, Saltzman W Mark
School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA.
Biomaterials. 2004 Mar-Apr;25(7-8):1331-7. doi: 10.1016/j.biomaterials.2003.08.013.
Poly-D-lysine, poly-L-lysine, collagen, laminin, fibronectin, and Matrigel were compared with standard tissue grade polystyrene for their impact on the expansion and neuronal differentiation of mesenchymal stem cells (MSCs). Among these substrates, adsorption of Matrigel at 5 microg/cm2 did not enhance cell proliferation but gave rise to the highest percentage of MSC-derived neuron-like cells with the best morphological differentiation. Matrigel at a higher coating density of 50 microg/cm2 not only further enhanced the differentiation but also significantly improved cell expansion. In contrast, poly-D-lysine did not effectively support the growth of MSCs. Hence the expansion and neuronal differentiation of MSCs both depend on surface properties of the culture substrate. These results could lead to a culture process with improved yield of MSC-derived neuron-like cells and to novel biomaterials for tissue engineering.
将聚-D-赖氨酸、聚-L-赖氨酸、胶原蛋白、层粘连蛋白、纤连蛋白和基质胶与标准组织级聚苯乙烯进行比较，以研究它们对间充质干细胞（MSC）扩增和神经元分化的影响。在这些底物中，5 μg/cm² 的基质胶吸附量虽未促进细胞增殖，但能产生最高比例的 MSC 来源的神经元样细胞，且形态分化最佳。50 μg/cm² 的较高包被密度的基质胶不仅进一步增强了分化，还显著促进了细胞扩增。相比之下，聚-D-赖氨酸不能有效支持 MSC 的生长。因此，MSC 的扩增和神经元分化均取决于培养底物的表面特性。这些结果可能会带来一种提高 MSC 来源的神经元样细胞产量的培养方法，并为组织工程带来新型生物材料。
