Standifer Nathan E, Kraig Ellen, Infante Anthony J
Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr, San Antonio, TX 78229-3900, USA.
J Neuroimmunol. 2003 Dec;145(1-2):68-76. doi: 10.1016/j.jneuroim.2003.09.007.
The predominant murine T lymphocyte population responding to Talpha146-162, the immunodominant epitope in EAMG, expresses the TCRBV 6 gene segment. However, cells expressing other TCRBV gene segments also react with this peptide. In order to more precisely characterize the Talpha146-162-specific TCR repertoire, we isolated CD4high cells from peptide-immunized mice. The majority of CD4high cells utilized an acidic TCR beta chain CDR3 motif regardless of TCRBV gene usage. Analysis of T cell clones demonstrated a fourfold higher avidity of Vbeta6+ than non-Vbeta6 cells for Talpha146-162 indicating that a hierarchy of TCR motifs determines T cell responsiveness in EAMG.
对实验性自身免疫性重症肌无力（EAMG）中的免疫显性表位Tα146 - 162产生应答的主要小鼠T淋巴细胞群体表达TCRBV 6基因片段。然而，表达其他TCRBV基因片段的细胞也会与该肽发生反应。为了更精确地表征Tα146 - 162特异性TCR库，我们从经肽免疫的小鼠中分离出CD4高细胞。无论TCRBV基因的使用情况如何，大多数CD4高细胞都利用一种酸性TCRβ链CDR3基序。对T细胞克隆的分析表明，Vβ6 +细胞对Tα146 - 162的亲和力比非Vβ6细胞高四倍，这表明TCR基序的层次结构决定了EAMG中的T细胞反应性。
