Yang Shuhua, Yu Hao, Xu Yifeng, Goh Chong Jin
Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Science Drive 4, 117543, Singapore.
FEBS Lett. 2003 Dec 4;555(2):291-6. doi: 10.1016/s0014-5793(03)01259-6.
The plant hormone cytokinin plays a major role in regulating plant growth and development. Here we generated cytokinin-reduction Arabidopsis plants by overexpressing a heterologous cytokinin oxidase gene DSCKX1 from Dendrobium orchid. These transgenic plants exhibited reduced biomass, rapid root growth, decreased ability to form roots in vitro, and reduced response to cytokinin in growing calli and roots. Furthermore, the expression of KNAT1, STM, and CycD3 genes was significantly reduced in the transgenic plants, suggesting that cytokinin may function to control the cell cycles and shoot/root development via regulation of these genes.
植物激素细胞分裂素在调节植物生长发育中起主要作用。在此，我们通过过表达来自石斛兰的异源细胞分裂素氧化酶基因DSCKX1，培育出了细胞分裂素含量降低的拟南芥植株。这些转基因植株表现出生物量减少、根系快速生长、体外生根能力下降以及愈伤组织和根在生长过程中对细胞分裂素的反应降低。此外，转基因植株中KNAT1、STM和CycD3基因的表达显著降低，这表明细胞分裂素可能通过调控这些基因来控制细胞周期和茎/根发育。
