Takanami Takako, Zhang Yongzhao, Aoki Hidetoshi, Abe Tomoko, Yoshida Shigeo, Takahashi Hideyuki, Horiuchi Saburo, Higashitani Atsushi
Graduate School of Life Sciences, Tohoku University, Saitama, Japan.
J Radiat Res. 2003 Sep;44(3):271-6. doi: 10.1269/jrr.44.271.
The effects of heavy ion particle irradiation on meiosis and reproductive development in the nematode Caenorhabditis elegans were studied. Meiotic pachytene nuclei are significantly resistant to particle irradiation by the heavy ions carbon and argon, as well as to X-rays, but not UV, whereas diplotene to diakinesis stage oocytes and early embryonic cells are not. Chromosomal abnormalities appear in mitotic cells and in maturing oocytes irradiated with heavy ion particles during the diplotene to the early diakinesis stages, but not in oocytes irradiated during the pachytene stage. The pachytene nuclei of ced-3 mutants, which are defective in apoptosis, are similarly resistant to ionizing radiation, but pachytene nuclei depleted for Ce-atl-1 (ataxia-telangiectasia like 1) or Ce-rdh-1/rad-51 are more sensitive. Pachytene nuclei thus appear to effectively repair heavy ion-induced DNA damage by the meiotic homologous recombination system.
研究了重离子粒子辐照对秀丽隐杆线虫减数分裂和生殖发育的影响。减数分裂粗线期细胞核对碳和氩重离子粒子辐照以及X射线具有显著抗性，但对紫外线不具有抗性，而双线期至终变期的卵母细胞和早期胚胎细胞则不然。在双线期至早期终变期阶段，用重离子粒子辐照的有丝分裂细胞和成熟卵母细胞中会出现染色体异常，但在粗线期辐照的卵母细胞中则不会出现。在细胞凋亡方面存在缺陷的ced-3突变体的粗线期细胞核同样对电离辐射具有抗性，但缺乏Ce-atl-1（共济失调毛细血管扩张样1）或Ce-rdh-1/rad-51的粗线期细胞核更敏感。因此，粗线期细胞核似乎能通过减数分裂同源重组系统有效修复重离子诱导的DNA损伤。
