糖皮质激素诱导肿瘤坏死因子受体配体的克隆与特性分析

Cloning and characterization of GITR ligand.

作者信息

Kim J D, Choi B K, Bae J S, Lee U H, Han I S, Lee H W, Youn B S, Vinay D S, Kwon B S

机构信息

Immunomodulation Research Center, University of Ulsan, Ulsan, Korea.

出版信息

Genes Immun. 2003 Dec;4(8):564-9. doi: 10.1038/sj.gene.6364026.

DOI:10.1038/sj.gene.6364026

PMID:14647196

Abstract

The gene encoding the natural ligand of murine glucocorticoid-induced tumor necrosis factor receptor (GITR) was cloned and characterized. The putative GITR ligand (GITRL) is composed of 173 amino acids with features resembling those of type II membrane proteins and is 51% identical to the human activation-inducible TNF receptor (AITR) ligand, TL6. Expression of the GITRL is restricted to immature and mature splenic dendritic cells. GITRL binds GITR expressed on HEK 293 cells and triggers NF-kappaB activation. Functional studies reveal that soluble CD8-GITRL prevents CD4+CD25+ regulatory T-cell-mediated suppressive activities.

摘要

编码小鼠糖皮质激素诱导的肿瘤坏死因子受体（GITR）天然配体的基因被克隆并进行了表征。推测的GITR配体（GITRL）由173个氨基酸组成，具有类似于II型膜蛋白的特征，并且与人类活化诱导型TNF受体（AITR）配体TL6有51%的同源性。GITRL的表达仅限于未成熟和成熟的脾树突状细胞。GITRL与HEK 293细胞上表达的GITR结合并触发NF-κB激活。功能研究表明，可溶性CD8-GITRL可阻止CD4+CD25+调节性T细胞介导的抑制活性。