Lanza F, Campioni D, Punturieri M, Moretti S, Dabusti M, Spanedda R, Castoldi G
Section of Hematology, Department of Biomedical Sciences and Advanced Therapies, University of Ferrara, Italy.
Bone Marrow Transplant. 2003 Dec;32(12):1165-73. doi: 10.1038/sj.bmt.1704279.
The distribution and functional characteristics of in vitro bone marrow (BM) endothelial colonies (CFU-En) were studied in 70 non-Hodgkin's lymphoma (NHL) patients in different phases of the disease to explore the association between CFU-En growth and angiogenesis, and between the number of CFU-En and the presence of hematopoietic and mesenchymal progenitor cells. The mean number of CFU-En/10(6) BM mononuclear cells seen in remission patients was significantly higher than that seen in newly diagnosed patients (P=0.04), and in normal subjects (P=0.008). Patients with low-grade NHL in remission displayed a higher CFU-En value compared with high-grade NHL (P=0.04). In the autograft group (40 patients), a significant reduction of CFU-En number was detected in the first 4-6 months after transplantation. In remission patients, the CFU-En number positively correlated with the incidence of BM colony-forming unit granulocyte-macrophage (CFU-GM) (P=0.013) and CFU-multilineage (CFU-GEMM) hematopoietic colonies (P=0.044). These in vitro data show that CFU-En numbers increase following standard-dose chemotherapy, thus providing a rationale for further investigating the effects of different cytostatic drugs on BM endothelial cells growth and function.
对70例处于疾病不同阶段的非霍奇金淋巴瘤（NHL）患者的体外骨髓（BM）内皮集落（CFU-En）的分布和功能特征进行了研究，以探讨CFU-En生长与血管生成之间以及CFU-En数量与造血和间充质祖细胞存在之间的关联。缓解期患者中每10⁶个BM单核细胞中CFU-En的平均数量显著高于新诊断患者（P = 0.04）和正常受试者（P = 0.008）。缓解期的低度NHL患者与高度NHL患者相比，CFU-En值更高（P = 0.04）。在自体移植组（40例患者）中，移植后前4至6个月检测到CFU-En数量显著减少。在缓解期患者中，CFU-En数量与BM集落形成单位粒细胞-巨噬细胞（CFU-GM）（P = 0.013）和CFU-多系（CFU-GEMM）造血集落的发生率呈正相关（P = 0.044）。这些体外数据表明，标准剂量化疗后CFU-En数量增加，从而为进一步研究不同细胞毒性药物对BM内皮细胞生长和功能的影响提供了理论依据。
