Nakatani Kazuki, Okuyama Hiroaki, Shimahara Yasuyuki, Saeki Shigeru, Kim Dong-Ho, Nakajima Yuji, Seki Shuichi, Kawada Norifumi, Yoshizato Katsutoshi
Department of Anatomy, Graduate School of Medicine, Osaka City University, Japan.
Lab Invest. 2004 Jan;84(1):91-101. doi: 10.1038/labinvest.3700013.
Cytoglobin/stellate cell activation-associated protein (Cygb/STAP) consists of a new class of hexacoordinate globin superfamily, which was recently discovered by a proteome analysis on the rat hepatic stellate cells. Unlike haemoglobin, myoglobin, and neuroglobin, Cygb/STAP is ubiquitously expressed in several organs, although its detailed localization has not been clarified. Immunohistochemistry and immunoelectron microscopy revealed that Cygb/STAP is uniquely localized in fibroblast-like cells in splanchnic organs, namely the vitamin A-storing cell lineage, but neither in epithelial cells, endothelial cells, muscle cells, blood cells, macrophages, nor dermal fibroblasts. The expression of Cygb/STAP was upregulated in fibrotic lesions of the pancreas and kidney in which activated fibroblast-like cells or myofibroblasts are known to increase in number. In cultured hepatic stellate cells, Cygb/STAP expression was augmented by the stimulation with sera, platelet-derived growth factor-BB, and transforming growth factor-beta 1. Overexpression of Cygb/STAP in NIH 3T3 cells induced the cells to lessen migratory activities and increase the expression of collagen alpha1(I) mRNA. These results indicate that Cygb/STAP is a tissue globin uniquely localized in splanchnic fibroblastic cell lineage and may play a role in fibrotic organ disorder.
细胞珠蛋白/星状细胞激活相关蛋白（Cygb/STAP）属于一类新的六配位珠蛋白超家族，它是最近通过对大鼠肝星状细胞的蛋白质组分析发现的。与血红蛋白、肌红蛋白和神经珠蛋白不同，Cygb/STAP在多个器官中普遍表达，尽管其详细定位尚未明确。免疫组织化学和免疫电子显微镜显示，Cygb/STAP独特地定位在内脏器官的成纤维细胞样细胞中，即储存维生素A的细胞系，但在上皮细胞、内皮细胞、肌肉细胞、血细胞、巨噬细胞或真皮成纤维细胞中均未发现。在胰腺和肾脏的纤维化病变中，Cygb/STAP的表达上调，已知在这些病变中活化的成纤维细胞样细胞或肌成纤维细胞数量增加。在培养的肝星状细胞中，血清、血小板衍生生长因子-BB和转化生长因子-β1刺激可增强Cygb/STAP的表达。在NIH 3T3细胞中过表达Cygb/STAP可诱导细胞减少迁移活性并增加胶原蛋白α1（I）mRNA的表达。这些结果表明，Cygb/STAP是一种独特地定位在内脏成纤维细胞系中的组织珠蛋白，可能在纤维化器官疾病中起作用。
