Liu Y C, Hayashi Y, Tohnai I, Kaneda T, Ohtsuka K
Department of Oral Surgery, Nagoya University School of Medicine, Japan.
J Radiat Res. 1992 Sep;33(3):199-210. doi: 10.1269/jrr.33.199.
We investigated intracellular translocation of hsp70 and total protein synthesis during heating at mild temperatures in NRK (normal rat kidney) cells. When cells were heated at 41 degrees C, the hsp70 was translocated into the nuclei/nucleoli, then returned gradually to the cytoplasm during heating. Concomitantly, total protein synthesis first decreased to 80% of the control level, then recovered by 2 hr. This indicates the acquisition of translational thermotolerance during heating at 41 degrees C. The return of hsp70 to the cytoplasm seems to be related to the recovery of total protein synthesis. Similar relationships were obtained at 42 degrees C heating in the presence of glycerol. These results suggest that translocation of hsp70 into the nuclei/nucleoli is important for the recovery of protein synthesis (acquisition of translational thermotolerance) during heating at mild temperatures.
我们研究了NRK（正常大鼠肾）细胞在温和温度加热过程中hsp70的细胞内转运及总蛋白合成情况。当细胞在41℃加热时，hsp70转运至细胞核/核仁，然后在加热过程中逐渐回到细胞质。与此同时，总蛋白合成首先降至对照水平的80%，然后在2小时内恢复。这表明在41℃加热过程中获得了翻译耐热性。hsp70回到细胞质似乎与总蛋白合成的恢复有关。在42℃加热且存在甘油的情况下也得到了类似的关系。这些结果表明，在温和温度加热过程中，hsp70转运至细胞核/核仁对于蛋白质合成的恢复（翻译耐热性的获得）很重要。
