Yfanti Eleni, Sidera Katerina, Margaritis Lukas H, Patsavoudi Evangelia
Department of Biochemistry, Hellenic Pasteur Institute, Athens, Greece.
Glia. 2004 Jan 1;45(1):39-53. doi: 10.1002/glia.10307.
The monoclonal antibody 4C5 recognizes a cell surface antigen of the developing central nervous system (CNS) and peripheral nervous system (PNS). In vitro antibody perturbation experiments have shown that the 4C5 antigen is involved in horizontal and vertical migration processes of granule cells during development of the rodent cerebellum. Moreover, results concerning the cellular localization and temporal expression of the 4C5 antigen during development and after injury of the rat sciatic nerve suggested that it may participate in Schwann cell migrations that occur during the above processes. To test this possibility, we examined the effects of our function-blocking antibody on Schwann cell migration in three in vitro bioassays: in tissue cultures from developing sciatic nerve, in dorsal root ganglion cultures on cryostat sections of normal or denervated adult sciatic nerve, and in pure Schwann cell cultures. The results showed that the presence of monoclonal antibody 4C5 in all the above culture systems strongly inhibited Schwann cell migration, indicating that the 4C5 antigen participates in migration processes that take place during development and regeneration of the peripheral nervous system. Moreover, staining of migrating Schwann cells in the presence of monoclonal antibody 4C5 with rhodamine-phalloidin showed that 4C5 antigen activity is associated with actin cytoskeletal organization of these cells, and more specifically with lamellipodia formation.
单克隆抗体4C5识别发育中的中枢神经系统（CNS）和外周神经系统（PNS）的一种细胞表面抗原。体外抗体干扰实验表明，4C5抗原在啮齿动物小脑发育过程中参与颗粒细胞的水平和垂直迁移过程。此外，关于大鼠坐骨神经发育和损伤后4C5抗原的细胞定位和时间表达的结果表明，它可能参与上述过程中发生的雪旺细胞迁移。为了验证这种可能性，我们在三种体外生物测定中检测了我们的功能阻断抗体对雪旺细胞迁移的影响：在发育中的坐骨神经组织培养物中、在正常或去神经支配的成年坐骨神经冷冻切片上的背根神经节培养物中以及在纯雪旺细胞培养物中。结果表明，上述所有培养系统中存在单克隆抗体4C5均强烈抑制雪旺细胞迁移，表明4C5抗原参与外周神经系统发育和再生过程中发生的迁移过程。此外，用罗丹明 - 鬼笔环肽对存在单克隆抗体4C5的迁移雪旺细胞进行染色显示，4C5抗原活性与这些细胞的肌动蛋白细胞骨架组织有关，更具体地说与片状伪足的形成有关。
