非离子表面活性剂对疏水作用色谱中蛋白质色谱行为的影响

Influence of nonionic surfactants on the chromatographic behaviour of proteins in hydrophobic interaction chromatography.

作者信息

Rukhadze Marina D, Sebiskveradze Maya V, Makharadze Teona G, Sidamonidze Nino Sh

机构信息

Laboratory of Neurobiology and Department of Chemistry, Tbilisi State University, Tbilisi 380028, Georgia.

出版信息

Biomed Chromatogr. 2003 Dec;17(8):538-42. doi: 10.1002/bmc.282.

DOI:10.1002/bmc.282

PMID:14648612

Abstract

The chromatographic characteristics of proteins in the presence of additives of nonionic surfactants Brij-35 and Tween-80 in the conditions of descending gradient of ammonium sulfate and phenyl-coated polymeric stationary phase were investigated. It was revealed that retention factors of proteins may be regulated by use of mentioned additives. The improvement of resolution is achieved for some hardly separated pairs of proteins, viz. albumin egg/albumin bovine, aldolase/tripsin. A reversion of the elution order is observed for tripsin/chymotrypsinogen A.

摘要

研究了在硫酸铵梯度下降和苯基涂层聚合物固定相条件下，非离子表面活性剂Brij-35和吐温80添加剂存在时蛋白质的色谱特性。结果表明，使用上述添加剂可调节蛋白质的保留因子。对于一些难以分离的蛋白质对，如卵清蛋白/牛血清白蛋白、醛缩酶/胰蛋白酶，分离度得到了提高。对于胰蛋白酶/胰凝乳蛋白酶原A，观察到洗脱顺序发生了反转。