Institute of Chemistry, Karl-Franzens-University, Heinrichstrasse 28, Central Polymer Laboratory/Molecular Characteristics, A-8010 Graz, Austria.
J Chromatogr A. 2010 Dec 24;1217(52):8222-9. doi: 10.1016/j.chroma.2010.10.118. Epub 2010 Nov 5.
It is shown, that amphiphilic polymers--such as polysorbates and fatty esters of polyethylene glycol can be separated by comprehensive two-dimensional liquid chromatography using a reversed phase column (under critical conditions for the polyoxyethylene chain) and a HILIC column, which may arranged in different order. The mobile phases in both dimensions can be 93-97 wt% acetone water. As the retention of higher esters on the reversed phase column is very strong, this column should be used as the first dimension. On the HILIC column all fractions elute within a reasonably short time (at a flow rate of 2.5 ml/min within 2 min). With a flow rate of 0.1 ml/min in the first dimension, a full separation can be achieved in 90 min.
结果表明，亲脂性聚合物（如聚山梨酯和聚乙二醇脂肪酸酯）可以通过使用反相柱（在聚氧乙烯链的临界条件下）和 HILIC 柱的二维液相色谱法分离，这两个柱子可以以不同的顺序排列。两个维度中的流动相都可以是 93-97wt%丙酮水。由于较高酯在反相柱上的保留非常强，因此该柱应作为第一维。在 HILIC 柱上，所有馏分都在合理短的时间内洗脱（在 2.5ml/min 的流速下在 2 分钟内）。在第一维中以 0.1ml/min 的流速，可以在 90 分钟内实现完全分离。
Zotero 插件