Li Yujuan, Bi Kaishun
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, People's Republic of China.
Biomed Chromatogr. 2003 Dec;17(8):543-6. doi: 10.1002/bmc.274.
An HPLC method is described for determination of ferulic acid in rat plasma. The concentration of ferulic acid in rat plasma was determined after deproteinization with acetonitrile using sulfamethoxazole as internal standard. Chromatographic separations were performed on a C(18) stationary phase with a mobile phase composed of acetonitrile-water (16:84, v/v) with 1% glacial acetic acid. The UV detection wavelength was set at 320 nm. The method was successfully applied to the determination of pharmacokinetic parameters in rat plasma after oral administration of Rhizoma Chuanxiong and and its compound preparation Suanzaoren decoctions. The calibration curve was linear over the range 0.0510-4.08 micro g/mL in rat plasma. Within-day and between-day precisions were less than 4.5% RSD. Mean recovery was determined as 96.9%. The limit of quantitation was 0.0510 micro g/mL. The pharmacokinetic parameters of the two preparations were different significantly (p < 0.05), which may attribute to the effects of other ingredients present in Suanzaoren decoction.
描述了一种用于测定大鼠血浆中阿魏酸的高效液相色谱法。以磺胺甲恶唑为内标，用乙腈对大鼠血浆进行脱蛋白处理后，测定阿魏酸在大鼠血浆中的浓度。在C(18)固定相上进行色谱分离，流动相由乙腈-水(16:84, v/v)和1%冰醋酸组成。紫外检测波长设定为320 nm。该方法成功应用于大鼠口服川芎及其复方制剂酸枣仁汤后血浆药代动力学参数的测定。大鼠血浆中校准曲线在0.0510 - 4.08 μg/mL范围内呈线性。日内和日间精密度RSD均小于4.5%。平均回收率为96.9%。定量限为0.0510 μg/mL。两种制剂的药代动力学参数有显著差异(p < 0.05)，这可能归因于酸枣仁汤中其他成分的影响。
