Scholz Stefan, Domaschke H, Kanamori A, Ostermann K, Rödel G, Gutzeit H O
Institut für Zoologie, Technische Universität Dresden, Dresden, Germany.
Mol Reprod Dev. 2004 Jan;67(1):15-8. doi: 10.1002/mrd.20012.
The gene germ cell-less (gcl) plays an important role in the early differentiation of germ cells in Drosophila. We isolated the gcl homolog of the model teleost medaka (Oryzias latipes) using degenerated primers and an ovary cDNA bank. The predicted amino acid sequence of medaka gcl showed 92, 68 and 31% overall identity to mouse, human and Drosophila gcl respectively. RT-PCR revealed stronger expression in the ovary and weaker expression in testis, brain, heart, liver and muscle tissue. Expression in early embryos indicates the presence of maternal mRNA. By in situ hybridisation (ISH), gcl could not be detected in embryos. In contrast to vasa, ISH revealed expression of gcl in the ovary but not in the testis. Mol. Reprod. Dev. 67: 15-18, 2004.
生殖细胞缺失基因（gcl）在果蝇生殖细胞的早期分化中起着重要作用。我们使用简并引物和卵巢cDNA文库分离了模式硬骨鱼青鳉（Oryzias latipes）的gcl同源物。青鳉gcl的预测氨基酸序列与小鼠、人类和果蝇的gcl的总体同源性分别为92%、68%和31%。逆转录聚合酶链反应（RT-PCR）显示，其在卵巢中的表达较强，而在睾丸、脑、心脏、肝脏和肌肉组织中的表达较弱。早期胚胎中的表达表明存在母体mRNA。通过原位杂交（ISH），在胚胎中未检测到gcl。与vasa不同，ISH显示gcl在卵巢中表达，但在睾丸中不表达。《分子生殖与发育》67: 15 - 18，2004年。
