[X射线照射促进大鼠脊髓损伤功能和结构恢复的研究]

[Study of X-irradiation to enhance the functional and structural recovery of the injured spinal cord of rat].

作者信息

Li Gang, Wang Jie-zhen, Li Xin-gang, Zhang Qing-lin, Jia De-ze, Gong Song-feng

机构信息

Department of Neurosurgery, QiLu Hospital, Shandong University, Jinan 250012, China.

出版信息

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2003 Oct;25(5):577-80.

PMID:14650162

Abstract

OBJECTIVE

To explore whether X-irradiation can enhance the functional and structural recovery of the injured spinal cord of rats.

METHODS

Seventy Sprague-Dawley rats received spinal cord injury by clip compression at the T2 level were randomly divided into two groups. The experimental group received X-irradiation at 14 days after injury, the control group did not receive X-irradiation. The functional tests were performed at day 3, 7, 14, 21, 28, 35, and 42 after irradiation including open field movement, inclined plane and pain withdrawal test. All injured rats were sacrificed at 43 days after injury and the injured spinal cords were taken out for histological tests.

RESULTS

Sixty-two rats met the experimental requirements among 70 injured rats, 32 rats in experimental group and 30 rats in control group. Statistically significant difference was achieved between two groups in open field movement and inclined plane (P < 0.01), but not for the pain withdrawal test. The edema and necrosis area of injured spinal cords of experimental group were less than those in control group, and the number of axons of experimental group were more than those in control group.

CONCLUSIONS

X-irradiation can enhance the functional recovery by improving and restoring structural integrity of the injured spinal cord.

摘要

目的

探讨X射线照射能否促进大鼠脊髓损伤后的功能和结构恢复。

方法

将70只经T2水平夹闭压迫造成脊髓损伤的Sprague-Dawley大鼠随机分为两组。实验组在损伤后14天接受X射线照射，对照组未接受X射线照射。在照射后第3、7、14、21、28、35和42天进行功能测试，包括旷场运动、斜面试验和疼痛退缩试验。所有损伤大鼠在损伤后43天处死，取出损伤脊髓进行组织学检查。