Carulli G, Gianfaldoni M L, Azzara A, Papineschi F, Vanacore R, Minnucci S, Testi R, Ambrogi F
Unit of Hematology, Medical Clinic 1, University of Pisa, Italy.
Leuk Res. 1992 Dec;16(12):1203-9. doi: 10.1016/0145-2126(92)90120-v.
FcRIII (CD16) expression on neutrophils from 17 patients with chronic myeloid leukemia (CML) was studied by flow cytometry using monoclonal antibodies. A variable proportion of CD16-negative neutrophils were found both in CML patients in chronic phase (3 out of 8 patients) and in CML patients in hematological remission (3 out of 9 patients). Neutrophils with reduced FcRIII expression showed more defective chemiluminescence and phagocytosis than neutrophils with normal FcRIII expression. Circulating myeloid cells from three patients in chronic phase, showing a normal percentage of CD16-positive neutrophils, were isolated and fractionated by discontinuous Percoll gradients. This study showed that CD16 appears at the stage of metamyelocyte, that band cells and segmented neutrophils display an identical pattern of membrane FcRIII, and that the fluorescence intensity shown by metamyelocytes is different from that displayed by more mature cells. The association between low FcRIII expression and function abnormality could be suggestive of a defect in CML neutrophil maturation.
运用单克隆抗体，通过流式细胞术研究了17例慢性髓性白血病（CML）患者中性粒细胞上的FcRIII（CD16）表达情况。在慢性期CML患者（8例中有3例）和血液学缓解期CML患者（9例中有3例）中均发现了不同比例的CD16阴性中性粒细胞。与FcRIII表达正常的中性粒细胞相比，FcRIII表达降低的中性粒细胞表现出更明显的化学发光和吞噬功能缺陷。分离了3例慢性期患者循环中的髓系细胞，这些细胞中CD16阳性中性粒细胞的百分比正常，并通过不连续的Percoll梯度进行分级分离。该研究表明，CD16出现在晚幼粒细胞阶段，杆状核细胞和分叶核中性粒细胞表现出相同的膜FcRIII模式，且晚幼粒细胞显示的荧光强度与更成熟细胞显示的不同。低FcRIII表达与功能异常之间的关联可能提示CML中性粒细胞成熟存在缺陷。
