Sytnik E S, Pariĭ A F, Komarnitskiĭ I K, Gleba Iu Iu, Kuchuk N V
Tsitol Genet. 2003 Sep-Oct;37(5):3-8.
New model system of plastid transformation has been proposed using a wild representative of Solanaceae family--S. sinuata. Earlier obtained cybrid plants N. tabacum (+ S. sinuata) were used for transformation experiments by PEG treatment of protoplasts with aadA gene that confers resistance to spectinomycin. Transformed S. sinuata plastome was transferred from N. tabacum (+ S. sinuata) cybrid to S. sinuata wild type plants by somatic hybridization. Molecular analysis of nuclear and mitochondrial DNA has been performed.
已提出使用茄科野生代表植物——深裂曼陀罗建立质体转化新模型系统。早期获得的烟草（+深裂曼陀罗）胞质杂种植物用于转化实验，通过聚乙二醇处理原生质体并导入赋予壮观霉素抗性的aadA基因。通过体细胞杂交将转化的深裂曼陀罗质体基因组从烟草（+深裂曼陀罗）胞质杂种转移到深裂曼陀罗野生型植物中。已对核DNA和线粒体DNA进行了分子分析。
