Western Robert J, Marriott Philip J
Australian Centre for Research on Separation Science, RMIT University, GPO Box 2476V, Melbourne 3001, Australia.
J Chromatogr A. 2003 Nov 26;1019(1-2):3-14. doi: 10.1016/j.chroma.2003.09.006.
Two methods of generating transportable second dimension retention data are outlined for comprehensive two-dimensional gas chromatography (GC x GC). They are both refinements of a previously outlined procedure, which adapted 'isovolatile' curves to retention prediction maps developed in our laboratory, extended to a more polar homologous series, the linear primary alcohols. The earlier work investigated maps based on alkanes and methyl ketones and methyl esters; here the method of data collection to generate the retention map has also changed, extending the retention base range. The resulting retention map permits a retention basis to be used for GC x GC data, however, a more polar retention set than alkanes is required. The calculation of 'retention indices' is aided by the generation of 'fractional reference compound' curves, by either direct manipulation of data local to the solute, or generation of a discrete curve coincident with the retention co-ordinates of the target compound.
本文概述了两种用于全二维气相色谱（GC x GC）生成可转移二维保留数据的方法。它们都是对先前概述程序的改进，该程序将“等挥发性”曲线应用于我们实验室开发的保留预测图，并扩展到更具极性的同系物系列——线性伯醇。早期的工作研究了基于烷烃、甲基酮和甲酯的图谱；在此，生成保留图谱的数据收集方法也发生了变化，扩展了保留基础范围。由此产生的保留图谱允许将保留基础用于GC x GC数据，然而，需要一组比烷烃更具极性的保留集。通过直接处理溶质局部的数据或生成与目标化合物保留坐标重合的离散曲线来生成“分数参考化合物”曲线，有助于“保留指数”的计算。
