Jahn H, Rose F, Schmitt R, Melin G, Schohn D, Comte G, Schaetzel S
Service de Néphrologie, Clinique médicale B, Hôpital Civil de Strasbourg, France.
Miner Electrolyte Metab. 1992;18(2-5):222-7.
The purpose of this study was to investigate the modifications of muscle protein synthesis activity in uremic patients fed a low-protein diet and a low-protein diet supplemented with a keto acid-amino acid mixture. The protein synthesis activity was evaluated in vitro on isolated muscle ribosomes incubated in a cell-free medium with tritiated leucine. Simultaneously, nitrogen kinetics and amino acid patterns were examined. Protein synthesis activity is correlated with the protein content of the diet in uremic patients. The keto acid-amino acid supplementation enhances protein synthesis. Variations of protein synthesis can be correlated with the variations of nitrogen balance which implies a major role of protein synthesis activity in muscle protein metabolism. Variations in plasma levels of the essential amino acids, mainly leucine and valine, can be correlated with the variations of protein synthesis activity, and these amino acids seem therefore to be mediators of the dietary effects on protein synthesis in uremia.
本研究的目的是调查接受低蛋白饮食以及补充了酮酸 - 氨基酸混合物的低蛋白饮食的尿毒症患者肌肉蛋白质合成活性的变化。在含有氚标记亮氨酸的无细胞培养基中孵育的分离肌肉核糖体上，对蛋白质合成活性进行体外评估。同时，检测氮动力学和氨基酸模式。尿毒症患者的蛋白质合成活性与饮食中的蛋白质含量相关。补充酮酸 - 氨基酸可增强蛋白质合成。蛋白质合成的变化与氮平衡的变化相关，这意味着蛋白质合成活性在肌肉蛋白质代谢中起主要作用。必需氨基酸（主要是亮氨酸和缬氨酸）血浆水平的变化与蛋白质合成活性的变化相关，因此这些氨基酸似乎是饮食对尿毒症患者蛋白质合成影响的介质。
