Martin A A, Tomas F M, Ballard F J, Read L C
Child Health Research Institute, North Adelaide, South Australia.
Miner Electrolyte Metab. 1992;18(2-5):264-8.
The growth-promoting effects of IGF-I were examined in rats following partial nephrectomy and compared with the effects of the des(1-3) variant of IGF-I. Four groups of rats were subjected to 5/6 nephrectomy (n = 8 per group) and treated for 7 days with IGF-I (0.9 or 2.2 mg/kg BW/day), des(1-3)IGF-I (0.9 mg/kg/BW/day), or vehicle (0.1 M acetic acid) administered subcutaneously by osmotic pump. A group of vehicle-treated, sham-operated control rats (n = 7) was included. Food utilization was significantly improved in all three peptide-treated groups, by 13-16% compared with the vehicle-treated nephrectomized group. Also, nitrogen balance was enhanced, particularly in the des(1-3)IGF-I group, in which nitrogen excretion was reduced by 24%, with the low- and high-dose IGF-I groups showing 16 and 18% reductions, respectively. Serum urea levels were significantly decreased, by 25%, in the des(1-3)IGF-I group, with 20 and 17% reductions being observed in the low- and high-dose IGF-I groups. Muscle protein degradation was found to be significantly attenuated with des(1-3)IGF-I treatment but was not significantly affected in the two IGF-I-treated groups. While carcass composition was not altered with IGF peptide treatment, absolute mass of protein in the carcass was improved in rats treated with the high dose of IGF-I. These results show that IGF-I or, more particularly, des(1-3)IGF-I may be efficacious in overcoming impaired growth in renal failure.
研究了胰岛素样生长因子-I（IGF-I）在大鼠部分肾切除后的促生长作用，并与IGF-I的去（1-3）变体的作用进行了比较。将四组大鼠进行5/6肾切除术（每组n = 8），并用IGF-I（0.9或2.2 mg/kg体重/天）、去（1-3）IGF-I（0.9 mg/kg体重/天）或通过渗透泵皮下给药的载体（0.1 M乙酸）治疗7天。包括一组接受载体治疗的假手术对照大鼠（n = 7）。与接受载体治疗的肾切除组相比，所有三个肽治疗组的食物利用率均显著提高，提高了13 - 16%。此外，氮平衡得到增强，特别是在去（1-3）IGF-I组，其中氮排泄减少了24%，低剂量和高剂量IGF-I组分别减少了16%和18%。去（1-3）IGF-I组的血清尿素水平显著降低了25%，低剂量和高剂量IGF-I组分别降低了20%和17%。发现去（1-3）IGF-I治疗可显著减轻肌肉蛋白质降解，但两个IGF-I治疗组未受到显著影响。虽然IGF肽治疗未改变胴体组成，但高剂量IGF-I治疗的大鼠胴体中蛋白质的绝对质量有所改善。这些结果表明，IGF-I，或更特别地，去（1-3）IGF-I可能有效克服肾衰竭时的生长受损。
