Zou Shujuan, Wang Zhiguo, Hu Jing
Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2003 Oct;21(5):342-3, 363.
To observe the expression patterns of matrix metalloproteinase-3 and its inhibitor, tissue inhibitor metalloproteinases (TIMP)-1, in remodeling phase of mandibular distraction osteogenesis.
Bilateral mandibular osteotomies were performed in 8 mature rabbits. The mandibles were lengthened 6 mm using a custom-made distractor with a rate of 1 mm/d. All animals were killed in 4 weeks after completion of distraction. The distracted calluses were harvested and processed for histology and immunohistochemistry of MMP-3 and TIMP-1.
Elevated expression of both MMP-3 and TIMP-1 was found in the distracted calluses resulting from mandibular lengthening. Positive staining for MMP-3 was seen in the osteoblasts and osteocytes, and TIMP-1 was mainly localized in osteoblasts.
MMP-3 and TIMP-1 appear to play important roles in the remodeling of new bone created by mandibular distraction osteogenesis.
观察基质金属蛋白酶-3及其抑制剂金属蛋白酶组织抑制剂（TIMP）-1在下颌骨牵张成骨重塑期的表达模式。
对8只成年兔进行双侧下颌骨截骨术。使用定制的牵张器以1mm/d的速度将下颌骨延长6mm。牵张完成后4周处死所有动物。收集牵张部位的骨痂，进行MMP-3和TIMP-1的组织学及免疫组织化学检测。
在下颌骨延长形成的牵张骨痂中发现MMP-3和TIMP-1的表达均升高。MMP-3在成骨细胞和骨细胞中呈阳性染色，而TIMP-1主要定位于成骨细胞。
MMP-3和TIMP-1似乎在下颌骨牵张成骨形成的新骨重塑中起重要作用。
