一种具有与野生绿头菇红菇独特特征的核糖核酸酶。
A ribonuclease with distinctive features from the wild green-headed mushroom Russulus virescens.
作者信息
Wang Hexiang, Ng Tzi Bun
机构信息
Department of Microbiology, College of Biological Science, China Agricultural University, and State Key Laboratory of Agrobiotechnology, Beijing, China.
出版信息
Biochem Biophys Res Commun. 2003 Dec 26;312(4):965-8. doi: 10.1016/j.bbrc.2003.10.201.
A ribonuclease with an N-terminal sequence different from those of other ribonucleases has been purified from fruiting bodies of the mushroom Russula virescens. The RNase was adsorbed on DEAE-cellulose and Q-Sepharose in 10mM Tris-HCl buffer (pH 7.1-7.3) and on CM-Sepharose in 10mM NH(4)OAc buffer (pH 4.6), unlike other mushroom ribonucleases which are unadsorbed on DEAE-cellulose. The RNase demonstrated a molecular mass of 28kDa in both gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In contrast to other mushroom ribonucleases which are monospecific, it exhibited co-specificity towards poly A and poly C. It demonstrated a pH optimum of 4.5, which is lower than values reported for other mushroom ribonucleases, and a temperature optimum of 60 degrees C.
一种N端序列与其他核糖核酸酶不同的核糖核酸酶已从绿红菇子实体中纯化出来。该核糖核酸酶在10mM Tris-HCl缓冲液（pH 7.1 - 7.3）中能吸附于DEAE-纤维素和Q-琼脂糖，在10mM NH₄OAc缓冲液（pH 4.6）中能吸附于CM-琼脂糖，这与其他蘑菇核糖核酸酶不吸附于DEAE-纤维素不同。在凝胶过滤和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中，该核糖核酸酶的分子量均显示为28kDa。与其他单一特异性的蘑菇核糖核酸酶不同，它对聚A和聚C表现出共同特异性。它的最适pH为4.5，低于其他蘑菇核糖核酸酶报道的值，最适温度为60℃。
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