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Directed evolution of the site specificity of Cre recombinase.
Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4185-90. doi: 10.1073/pnas.022039799. Epub 2002 Mar 19.
4
Sequence of the loxP site determines the order of strand exchange by the Cre recombinase.
J Mol Biol. 2003 Feb 14;326(2):397-412. doi: 10.1016/s0022-2836(02)01429-8.
8
Cre mutants with altered DNA binding properties.
J Biol Chem. 1998 Sep 4;273(36):22884-91. doi: 10.1074/jbc.273.36.22884.
9
Mechanisms of Cre recombinase synaptic complex assembly and activation illuminated by Cryo-EM.
Nucleic Acids Res. 2022 Feb 22;50(3):1753-1769. doi: 10.1093/nar/gkac032.
10
Alteration of Cre recombinase site specificity by substrate-linked protein evolution.
Nat Biotechnol. 2001 Nov;19(11):1047-52. doi: 10.1038/nbt1101-1047.

引用本文的文献

1
Mechanisms of Cre recombinase synaptic complex assembly and activation illuminated by Cryo-EM.
Nucleic Acids Res. 2022 Feb 22;50(3):1753-1769. doi: 10.1093/nar/gkac032.
2
DNA binding induces a -to- switch in Cre recombinase to enable intasome assembly.
Proc Natl Acad Sci U S A. 2020 Oct 6;117(40):24849-24858. doi: 10.1073/pnas.2011448117. Epub 2020 Sep 23.
4
A heterodimer of evolved designer-recombinases precisely excises a human genomic DNA locus.
Nucleic Acids Res. 2020 Jan 10;48(1):472-485. doi: 10.1093/nar/gkz1078.
5
High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases.
Nat Commun. 2019 Apr 26;10(1):1937. doi: 10.1038/s41467-019-09987-0.
6
Engineering altered protein-DNA recognition specificity.
Nucleic Acids Res. 2018 Jun 1;46(10):4845-4871. doi: 10.1093/nar/gky289.
7
Crystal structure of an engineered, HIV-specific recombinase for removal of integrated proviral DNA.
Nucleic Acids Res. 2017 Sep 19;45(16):9726-9740. doi: 10.1093/nar/gkx603.
9
Protein-DNA binding: complexities and multi-protein codes.
Nucleic Acids Res. 2014 Feb;42(4):2099-111. doi: 10.1093/nar/gkt1112. Epub 2013 Nov 16.
10
Expanding the scope of site-specific recombinases for genetic and metabolic engineering.
Biotechnol Bioeng. 2014 Jan;111(1):1-15. doi: 10.1002/bit.25096. Epub 2013 Sep 13.

本文引用的文献

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Processing of X-ray diffraction data collected in oscillation mode.
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
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The CCP4 suite: programs for protein crystallography.
Acta Crystallogr D Biol Crystallogr. 1994 Sep 1;50(Pt 5):760-3. doi: 10.1107/S0907444994003112.
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Non-contact positions impose site selectivity on Cre recombinase.
Nucleic Acids Res. 2002 Jul 1;30(13):2764-71. doi: 10.1093/nar/gkf399.
7
Directed evolution of the site specificity of Cre recombinase.
Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4185-90. doi: 10.1073/pnas.022039799. Epub 2002 Mar 19.
9
Alteration of Cre recombinase site specificity by substrate-linked protein evolution.
Nat Biotechnol. 2001 Nov;19(11):1047-52. doi: 10.1038/nbt1101-1047.

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