Pikulski Michael, Brodbelt Jennifer S
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712-0165, USA.
J Am Soc Mass Spectrom. 2003 Dec;14(12):1437-53. doi: 10.1016/j.jasms.2003.07.002.
The elucidation of flavonoid isomers is accomplished by electrospray ionization tandem mass spectrometry (ESI-MS/MS) via formation and collisional activated dissociation (CAD) of metal/flavonoid complexes containing an auxiliary ligand. Addition of a metal salt and a suitable neutral auxiliary ligand to flavonoids in solution results in the formation of [M(II) (flavonoid-H) ligand]+ complexes by ESI which, upon collisional activated dissociation, often result in more distinctive fragmentation patterns than observed for conventional protonated or deprotonated flavonoids. Previously, 2,2'-bipyridine was used as an auxiliary ligand, and now we compare and explore the use of alternative pyridyl ligands, including 4,7-diphenyl-1,10-phenanthroline. Using this technique, three groups of flavonoid glycoside isomers are differentiated, including glycosides of apigenin, quercetin, and luteolin.
通过电喷雾电离串联质谱法（ESI-MS/MS），经由包含辅助配体的金属/类黄酮配合物的形成及碰撞活化解离（CAD）来完成类黄酮异构体的解析。向溶液中的类黄酮添加金属盐和合适的中性辅助配体，通过电喷雾电离会形成[M(II) (类黄酮-H) 配体]+配合物，这些配合物在碰撞活化解离时，往往会产生比传统质子化或去质子化类黄酮更独特的碎片模式。此前，2,2'-联吡啶被用作辅助配体，现在我们比较并探索包括4,7-二苯基-1,10-菲咯啉在内的替代吡啶基配体的用途。利用该技术，可以区分三组类黄酮糖苷异构体，包括芹菜素、槲皮素和木犀草素的糖苷。
