Gambella G R, Bellotti S, Cutolo M, Modesti A, Scarpa S, Novelli A, Ravera G B
Istituto di patologia generale, Università di Genova, Italy.
Pathologica. 1992 May-Jun;84(1091):343-61.
Previous our studies showed that some steroid hormones, as pure crystalline Progesterone (pPc) and 17-alpha-hydroxyprogesterone capronate (17 alpha HPC) heightened the cirrhogenic action produced in rat liver by carbon tetrachloride. Medroxyprogesterone (MPA), however, did not appear to promote cirrhosis, but increased just steatosis. In the present paper, we have studied the above mentioned steroid hormones for their possible capability of inducing changes in plasma fibronectin concentration. For this purpose, the soluble plasma fibronectin level was measured in female rats 45 days after CCl4-induced cirrhosis, and it was compared with the insoluble fibronectin of liver (detected by immunostaining) and the collagen content in the organ. The results obtained show that, after treatment with CCl4 and MPA, both plasma and liver fibronectin content strongly increases, whereas liver collagen content lowers. However, after treatment with CCl4 alone or in association with the other two steroid hormones, any changes in fibronectin content is not observable, but, on the contrary, is evident a heightened collagen production associated with a cirrhotic change of liver.
我们之前的研究表明，一些甾体激素，如纯结晶孕酮（pPc）和己酸17-α-羟孕酮（17αHPC），会增强四氯化碳在大鼠肝脏中产生的致肝硬化作用。然而，甲羟孕酮（MPA）似乎并未促进肝硬化，而只是增加了脂肪变性。在本文中，我们研究了上述甾体激素诱导血浆纤连蛋白浓度变化的可能能力。为此，在四氯化碳诱导肝硬化45天后的雌性大鼠中测量了可溶性血浆纤连蛋白水平，并将其与肝脏中的不溶性纤连蛋白（通过免疫染色检测）以及器官中的胶原蛋白含量进行了比较。获得的结果表明，在用四氯化碳和甲羟孕酮治疗后，血浆和肝脏纤连蛋白含量均大幅增加，而肝脏胶原蛋白含量降低。然而，在用四氯化碳单独治疗或与其他两种甾体激素联合治疗后，未观察到纤连蛋白含量有任何变化，相反，明显出现了与肝脏肝硬化变化相关的胶原蛋白生成增加。
