芍药苷对小鼠海马CA1神经元钠电流的阻断作用。
Blockade of paeoniflorin on sodium current in mouse hippocampal CA1 neurons.
作者信息
Zhang Guang-Qin, Hao Xue-Mei, Chen Shi-Zhong, Zhou Pei-Ai, Cheng He-Ping, Wu Cai-Hong
机构信息
State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
出版信息
Acta Pharmacol Sin. 2003 Dec;24(12):1248-52.
AIM
To study the blockade of paeoniflorin (Pae) on I(Na) in the acutely isolated hippocampus neurons of mice.
METHODS
The whole-cell patch clamp technique was used.
RESULTS
Pae inhibited I(Na) in frequency-dependent and concentration-dependent manners, with an IC50 of 271 micromol/L. Pae 0.3 mmol/L shifted the activation potential of the maximal I(Na) from -40 mV to -30 mV, shifted the steady-state activation and inactivation curves toward more positive and negative potentials by 10.8 mV, and 18.2 mV, respectively, and postponed the recovery of I(Na) inactivation state from (4.2+/-0.7) ms to (9.8+/-1.2) ms.
CONCLUSION
Pae inhibited I(Na) in mouse hippocampus neurons.
目的
研究芍药苷（Pae）对急性分离的小鼠海马神经元中钠电流（I(Na)）的阻断作用。
方法
采用全细胞膜片钳技术。
结果
Pae以频率依赖性和浓度依赖性方式抑制I(Na)，半数抑制浓度（IC50）为271微摩尔/升。0.3毫摩尔/升的Pae使最大I(Na)的激活电位从-40毫伏移至-30毫伏，使稳态激活曲线和失活曲线分别向更正和更负的电位移动10.8毫伏和18.2毫伏，并将I(Na)失活状态的恢复时间从（4.2±0.7）毫秒推迟至（9.8±1.2）毫秒。
结论
Pae抑制小鼠海马神经元中的I(Na)。
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