Sun Rong, Yi Yin-Ping, Lv Li-Li, Zhang Zuo-Ping, Sun Hu, Liu Guo-Qing
Shandong Academy of Chinese Medicine, Jinan 250014, China.
Zhongguo Zhong Yao Za Zhi. 2007 Dec;32(23):2518-22.
To explore the effects of paeoniflorin on blood brain barrier and pathological changes in brain ischemia.
Mice were divided into sham operation group, model group, positive control group-Deng zhanhua tablet group and three different dose (high, middle, low-dose) groups of paeoniflorin. The neurological symptoms of rats were observed. The SOD of ischemic brain tissue, MDA BBB and EAA contents were determined. The ultrastructure on the brain cells was inspected by transmission electron microscope.
Paeoniflorin had the protetive effects on 4VO model of total cerebral ischemia. Paeoniflorin could obviously increase SOD content, reduce MDA content in rat brain-tissue and alleviate oxidative stress damage by cerebral ischemia on rat brain. Paeoniflorin could improve pathological changes of cell nuclear, perikaryon, mitochondria and myelin sheath, which was the morphologic basis of the protective effect on ischemia. Paeoniflorin could alleviate the incrense of EAA content caused by and hypoxia and inhibit the excitatory neural toxicity by EAA.
Paeoniflorin has the protection effect on the brain edema after cerebral ischemia, the oxidative stress damage on brain tissue, the ultrastructure lesions of cells and the BBB. The protective mechanism may be related to inhibiting intracellular calcium overload, anti-free radical and reducing EAA content.
探讨芍药苷对血脑屏障及脑缺血病理变化的影响。
将小鼠分为假手术组、模型组、阳性对照组（灯盏花素片组）和芍药苷三个不同剂量（高、中、低剂量）组。观察大鼠神经症状。测定缺血脑组织超氧化物歧化酶（SOD）、丙二醛（MDA）、血脑屏障及兴奋性氨基酸（EAA）含量。通过透射电子显微镜观察脑细胞超微结构。
芍药苷对全脑缺血4VO模型具有保护作用。芍药苷可明显增加大鼠脑组织中SOD含量，降低MDA含量，减轻脑缺血对大鼠脑的氧化应激损伤。芍药苷可改善细胞核、核周体、线粒体及髓鞘的病理变化，这是其对缺血具有保护作用的形态学基础。芍药苷可减轻缺氧所致EAA含量升高，抑制EAA的兴奋性神经毒性。
芍药苷对脑缺血后脑水肿、脑组织氧化应激损伤、细胞超微结构损伤及血脑屏障具有保护作用。其保护机制可能与抑制细胞内钙超载、抗自由基及降低EAA含量有关。
