Ono H, Klein D, Vincek V, Figueroa F, O'hUigin C, Tichy H, Klein J
Max-Planck-Institut für Biologie, Abteilung Immunogenetik, Tübingen, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11886-90. doi: 10.1073/pnas.89.24.11886.
Twenty cDNA clones derived from beta-chain-encoding class II genes of the zebrafish (Brachydanio rerio) major histocompatibility complex (MHC) have been sequenced. They fall into three groups identifying three loci of expressed genes. The length and organization of these genes are similar to those of their mammalian homologs. Amplification by polymerase chain reaction and sequencing of genomic DNA from zebrafish collected at different locations in India indicate the existence of a fourth group of sequences (fourth locus). A high degree of polymorphism at the B. rerio MHC loci and concentration of variability to the putative peptide-binding region of the beta 1-domain-encoding part of the gene are also indicated. Large genetic distances between alleles suggest trans-specific evolution of fish MHC polymorphism. Zebrafish genes appear to be derived from a different ancestor than the various class II gene families of other vertebrates. In spite of great sequence divergence between fish and mammalian MHC genes, there seems to be a striking conservation in their overall organization.
对来自斑马鱼（短担尼鱼）主要组织相容性复合体（MHC）Ⅱ类基因β链编码区的20个cDNA克隆进行了测序。它们分为三组，确定了三个表达基因位点。这些基因的长度和结构与其哺乳动物同源基因相似。通过聚合酶链反应扩增以及对从印度不同地点采集的斑马鱼基因组DNA进行测序，表明存在第四组序列（第四位点）。还表明短担尼鱼MHC位点存在高度多态性，且变异性集中在该基因β1结构域编码部分的假定肽结合区域。等位基因之间的大遗传距离表明鱼类MHC多态性的跨物种进化。斑马鱼基因似乎起源于与其他脊椎动物的各种Ⅱ类基因家族不同的祖先。尽管鱼类和哺乳动物的MHC基因之间存在很大的序列差异，但它们的整体结构似乎有显著的保守性。
