Kańczuga-Koda Luiza, Sulkowska Mariola, Koda Mariusz, Reszeć Joanna, Famulski Waldemar, Baltaziak Marek, Sulkowski Stanisław
Department of Clinical Pathology, Medical University, Białystok, Poland.
Folia Morphol (Warsz). 2003 Nov;62(4):439-42.
Gap junctional intercellular communication (GJIC) plays a critical role in tissue development and differentiation and probably in carcinogenesis. The purpose of the study was to evaluate the expression and localisation of Cx43 in 40 cases of mammary dysplasia and 29 cases of breast cancer (without primary chemotherapy). The tissue sections were investigated for Cx43 expression by immunohistochemistry. In the normal mammary gland there was an intercellular, punctate staining pattern, mainly between myoepithelial cells, characteristic of functional gap junctions. In dysplasias there was mainly mixed (cytoplasmic and intercellular) staining and in most cases of breast cancer we observed diffuse or granular, but cytoplasmic, staining of Cx43. Our results demonstrated that expression of Cx43 in dysplasias and breast cancer is changed and GJIC is probably impaired because of disruption of functional gap junction formation especially between breast cancer cells.
缝隙连接细胞间通讯（GJIC）在组织发育、分化以及可能在致癌过程中发挥着关键作用。本研究的目的是评估40例乳腺发育异常和29例乳腺癌（未接受原发化疗）中Cx43的表达和定位。通过免疫组织化学对组织切片进行Cx43表达研究。在正常乳腺中，存在细胞间点状染色模式，主要位于肌上皮细胞之间，这是功能性缝隙连接的特征。在发育异常中，主要是混合性（细胞质和细胞间）染色，而在大多数乳腺癌病例中，我们观察到Cx43呈弥漫性或颗粒状，但为细胞质染色。我们的结果表明，乳腺发育异常和乳腺癌中Cx43的表达发生了改变，并且由于功能性缝隙连接形成的破坏，尤其是乳腺癌细胞之间的破坏，GJIC可能受损。
