Słuczanowska-Głabowska Sylwia, Laszczyńska Maria, Wylot Marcin, Piasecka Małgorzata, Kram Andrzej
Department of Physiology, Pomeranian Medical University, Szczecin, Poland.
Folia Morphol (Warsz). 2003 Nov;62(4):501-3.
The effect of hyperprolactinaemia on the prostate has still not been fully elucidated. The aim of the study was to estimate the influence of hyperprolactinaemia on expression of the androgen receptor (AR) in rat epithelial cells of the prostate lateral lobe and on the morphology of these cells. Studies were performed on sexually mature male Wistar rats. To provoke hyperprolactinaemia rats received i.p. metoclopramid (MCP). For light and electron microscopy the lateral lobes were obtained routinely. The intensity of the immunohistochemical reaction of AR (expression of AR) in the epithelial cells of the prostate lateral lobe was assessed by optical density measurements with the help of computer image analysis. Ultrastructural observations of the epithelial cells of the lateral lobe were carried out by means of transmission and scanning electron microscopes. The results showed a more than twofold increase in prolactin (PRL) concentration in the serum, but a twofold decrease in testosterone (T). The intensity of the immunohistochemical reaction of AR in the epithelial cells of the lateral lobe in the experimental group was higher than in the control group. We noted changes in the morphology of the epithelial cells of the prostate lateral lobe in the experimental group.
高催乳素血症对前列腺的影响尚未完全阐明。本研究的目的是评估高催乳素血症对大鼠前列腺侧叶上皮细胞中雄激素受体（AR）表达及这些细胞形态的影响。研究对象为性成熟雄性Wistar大鼠。为诱发高催乳素血症，给大鼠腹腔注射甲氧氯普胺（MCP）。常规获取前列腺侧叶用于光镜和电镜检查。借助计算机图像分析通过光密度测量评估前列腺侧叶上皮细胞中AR免疫组化反应的强度（AR的表达）。通过透射电子显微镜和扫描电子显微镜对侧叶上皮细胞进行超微结构观察。结果显示血清中催乳素（PRL）浓度增加了两倍多，但睾酮（T）降低了两倍。实验组前列腺侧叶上皮细胞中AR免疫组化反应的强度高于对照组。我们注意到实验组前列腺侧叶上皮细胞的形态发生了变化。
