Ryu Se-Ah, Kim Chang Sup, Kim Hye-Jung, Baek Dae Heoun, Oh Deok-Kun
Department of Bioscience and Biotechnology, Sejong University, Seoul 143-747, Korea.
Biotechnol Prog. 2003 Nov-Dec;19(6):1643-7. doi: 10.1021/bp0340739.
D-Tagatose was continuously produced using thermostable L-arabinose isomerase immobilized in alginate with D-galactose solution in a packed-bed bioreactor. Bead size, L/D (length/diameter) of reactor, dilution rate, total loaded enzyme amount, and substrate concentration were found to be optimal at 0.8 mm, 520/7 mm, 0.375 h(-1), 5.65 units, and 300 g/L, respectively. Under these conditions, the bioreactor produced about 145 g/L tagatose with an average productivity of 54 g tagatose/L x h and an average conversion yield of 48% (w/w). Operational stability of the immobilized enzyme was demonstrated, with a tagatose production half-life of 24 days.
使用固定在藻酸盐中的热稳定L-阿拉伯糖异构酶和D-半乳糖溶液，在填充床生物反应器中连续生产D-塔格糖。发现珠子大小、反应器的长径比（长度/直径）、稀释率、总负载酶量和底物浓度分别在0.8毫米、520/7毫米、0.375 h⁻¹、5.65单位和300克/升时为最佳。在这些条件下，生物反应器产生了约145克/升的塔格糖，平均生产率为54克塔格糖/升·小时，平均转化率为48%（w/w）。证明了固定化酶的操作稳定性，塔格糖生产半衰期为24天。
