利用从嗜热脱硫杆菌中纯化得到的 L-阿拉伯糖异构酶和甘露糖-6-磷酸异构酶生产 L-核糖。
L-ribose production from L-arabinose by using purified L-arabinose isomerase and mannose-6-phosphate isomerase from Geobacillus thermodenitrificans.
机构信息
Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
出版信息
Appl Environ Microbiol. 2009 Nov;75(21):6941-3. doi: 10.1128/AEM.01867-09. Epub 2009 Sep 11.
Abstract
Two enzymes, L-arabinose isomerase and mannose-6-phosphate isomerase, from Geobacillus thermodenitrificans produced 118 g/liter L-ribose from 500 g/liter L-arabinose at pH 7.0, 70 degrees C, and 1 mM Co(2+) for 3 h, with a conversion yield of 23.6% and a volumetric productivity of 39.3 g liter(-1) h(-1).
摘要
两种酶，来自嗜热地芽孢杆菌的 L-阿拉伯糖异构酶和甘露糖-6-磷酸异构酶，在 pH 值为 7.0、70 摄氏度和 1mM Co(2+)的条件下，3 小时内将 500g/L 的 L-阿拉伯糖转化为 118g/L 的 L-核糖，转化率为 23.6%，体积产率为 39.3g 升(-1)小时(-1)。
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