Buitrón Germán, Betancur Manuel J, Moreno Gloria, Moreno Jaime A
Environmental Bioprocesses Department, Institute of Engineering, National University of Mexico, Ap. Postal 70-472, 04510 México D.F., Mexico.
Biotechnol Prog. 2003 Nov-Dec;19(6):1822-7. doi: 10.1021/bp0340842.
Combined anaerobic-aerobic processes are a viable alternative for the treatment of xenobiotic compounds that are difficult to treat by traditional processes. The variable nature of the sequencing batch reactors, SBR, systems allows manipulation of the selective pressure on the microorganisms. Then, the activity of the community can be dynamically adjusted to meet changing effluents conditions. To improve the response of the SBR to changing influent conditions, several efforts have been made to automate and control the duration of the sequential phases of the SBR. The objective of this work is to present and discuss the feasibility of the use of the oxidation-reduction potential, ORP, as a control variable for the determination of the anaerobic phase length in an anaerobic-aerobic SBR used to degrade p-nitrophenol, PNP. The control of the anaerobic phase of the anaerobic-aerobic reactor was achieved with software developed at the Institute of Engineering-UNAM. During the anaerobic stage, the PNP is reduced to p-aminophenol, PAP. As a consequence of the compound transformation, there is a change in the oxidation-reduction potential of the culture medium. This change was used to indicate the minimal concentration of PNP and, as a consequence, the maximal PAP concentration. The feasibility of the algorithm for using the variations in the ORP to determine on-line the length of the anaerobic stage in an anaerobic-aerobic process was demonstrated in our laboratory.
厌氧-好氧联合工艺是处理传统工艺难以处理的外源化合物的一种可行替代方法。序批式反应器（SBR）系统的可变性质允许对微生物的选择压力进行调控。这样，群落的活性就能动态调整以适应不断变化的出水条件。为了提高SBR对变化的进水条件的响应能力，人们已做出多项努力来实现SBR连续阶段持续时间的自动化和控制。这项工作的目的是介绍和讨论使用氧化还原电位（ORP）作为控制变量来确定用于降解对硝基苯酚（PNP）的厌氧-好氧SBR中厌氧阶段时长的可行性。厌氧-好氧反应器的厌氧阶段控制是通过国立自治大学工程学院开发的软件实现的。在厌氧阶段，PNP被还原为对氨基苯酚（PAP）。由于化合物的转化，培养基的氧化还原电位发生变化。这种变化被用来指示PNP的最低浓度，进而指示PAP的最高浓度。我们实验室已证明了利用ORP变化在线确定厌氧-好氧工艺中厌氧阶段时长的算法的可行性。
