Guo Y, Törkvist L, Raud J, Hedqvist P
Department of Physiology & Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Acta Physiol Scand. 2003 Dec;179(4):399-405. doi: 10.1046/j.0001-6772.2003.01219.x.
The role of mast cells and their principal mediator, histamine, in surgical skin flap survival was investigated using mast cell-deficient (Ws/Ws); their congenic littermates wild-type (+/+), and Wistar rats.
A standardized dorsal skin flap was raised and sutured back into position, and 6 days later the percentage of flap survival was assessed. Moreover, endogenous histamine concentration in the dorsal skin during the surgical preparation was determined using in vivo microdialysis technique together with high performance liquid chromatography-fluorometry. Accumulation of skin flap myeloperoxidase (MPO) (reflecting leucocyte recruitment) was determined spectrophotometrically.
The experimental skin flaps in genetically mast cell-deficient rats exhibited increased tissue survival and showed little accumulation of MPO and rather low and stable level of histamine output in comparison with skin flaps in the wild-type (+/+) littermates or normal Wistar rats. Antihistamine treatment inhibited but did not prevent leucocyte recruitment in the skin flaps post-surgery in +/+ and Wistar rats.
It is suggested that mast cell derived histamine plays an important role in leucocyte recruitment in skin flaps. However, mast cell-independent factors should be taken into consideration and needs further investigation as even in mast cell-deficient animals there was some accumulation of leucocytes and tissue necrosis in the skin flaps post-surgery.
使用肥大细胞缺陷型（Ws/Ws）大鼠、其同基因野生型（+/+） littermates 大鼠以及Wistar大鼠，研究肥大细胞及其主要介质组胺在手术皮瓣存活中的作用。
掀起一块标准化的背部皮瓣并缝合回原位，6天后评估皮瓣存活百分比。此外，在手术准备期间，使用体内微透析技术结合高效液相色谱 - 荧光法测定背部皮肤中内源性组胺浓度。用分光光度法测定皮瓣髓过氧化物酶（MPO）（反映白细胞募集）的积累。
与野生型（+/+） littermates 大鼠或正常Wistar大鼠的皮瓣相比，基因缺陷型肥大细胞大鼠的实验性皮瓣显示出组织存活率增加，MPO积累很少，组胺输出水平相当低且稳定。抗组胺治疗抑制但未阻止 +/+ 和Wistar大鼠术后皮瓣中的白细胞募集。
提示肥大细胞衍生的组胺在皮瓣白细胞募集中起重要作用。然而，应考虑肥大细胞非依赖性因素，并且需要进一步研究，因为即使在肥大细胞缺陷动物中，术后皮瓣中也有一些白细胞积累和组织坏死。
