对多发性硬化症患者进行干扰素-β（IFN-β）抗体的系列免疫沉淀试验。
Serial immunoprecipitation assays for interferon--(IFN)-beta antibodies in multiple sclerosis patients.
作者信息
Lampasona Vito, Rio Jordi, Franciotta Diego, Furlan Roberta, Avolio Carlo, Fazio Raffaella, Lavolpe Vito, Vincent Angela, Comi Giancarlo, Trojano Maria, Montalban Xavier, Martino Gianvito
机构信息
Department of Clinical Chemistry, San Raffaele Scientific Institute, Milan, Italy.
出版信息
Eur Cytokine Netw. 2003 Jul-Sep;14(3):154-7.
We devised a sensitive, radioimmunoprecipitation assay (RIPA) for anti-interferon (IFN)-beta-binding antibody (BAB) detection. Our RIPA showed good agreement with a reference RIPA (mean difference, -3.2 +/- 10.6 AU), and detected BAB to both IFN-beta-1a and IFN-beta-1b. Neutralizing antibodies to IFN-b (NAB) were also determined with a standard method. BAB and NAB were measured in 393 serum samples from 77 multiple sclerosis (MS) patients treated with IFN-beta-1a or -1b, who were studied over two years, and subsequently classified as responders and non-responders. BAB were found at higher concentrations, and more frequently detected, in IFN-beta-1b- than in IFN-beta-1a-treated patients, and, at highest titres, preferentially in patients who were positive for NAB. However, in our series of MS patients, both titres and frequency of detection of BAB or NAB did not differ between IFN-b responders and non-responders.
我们设计了一种用于检测抗干扰素（IFN）-β结合抗体（BAB）的灵敏的放射免疫沉淀测定法（RIPA）。我们的RIPA与参考RIPA显示出良好的一致性（平均差异为-3.2±10.6 AU），并且能检测到针对IFN-β-1a和IFN-β-1b的BAB。还采用标准方法测定了针对IFN-β的中和抗体（NAB）。在接受IFN-β-1a或-1b治疗的77例多发性硬化症（MS）患者的393份血清样本中测量了BAB和NAB，这些患者接受了两年的研究，随后被分类为反应者和无反应者。在接受IFN-β-1b治疗的患者中，BAB的浓度更高，检测频率也更高，并且在最高滴度时，优先出现在NAB呈阳性的患者中。然而，在我们的MS患者系列中，IFN-β反应者和无反应者之间BAB或NAB的滴度和检测频率并无差异。
Zotero 插件