Owczarzy Richard, Dunietz Isard, Behlke Mark A, Klotz Irving M, Walder Joseph A
Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA.
Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14840-5. doi: 10.1073/pnas.2335948100. Epub 2003 Dec 1.
Thermodynamic formulations have been devised to obtain Delta G degrees values directly from spectroscopic data at a fixed common temperature in nucleic acid duplex-simplex melting curves. In addition, the dependence of melting on salt concentration has been expressed in terms of a stepwise stoichiometric representation, which leads to a specific equation for the partition of the added sodium ions between the different oligonucleotide forms.