[肠道缺血/再灌注损伤对瘦素浓度影响的初步研究]
[Primary research of the effect of intestinal ischemia/reperfusion injury on Leptin concentrations].
作者信息
Lin Ji, Yan Guang-tao, Hao Xiu-hua, Zhang Kai, Wang Lu-huan, Xue Hui
机构信息
Research Laboratory of Biochemistry, Basic Medical Institute, General Hospital of People's Liberation Army, Beijing 100853, China.
出版信息
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2003 Dec;15(12):722-5.
OBJECTIVE
To explore the effect of intestinal ischemia/reperfusion injury on Leptin concentrations in serum and adipose tissue, and to find out the role of Leptin in acute inflammatory responses.
METHODS
An intestinal ischemia-reperfusion injury model of rats was established, and used a highly-sensitive murine Leptin radioimmunoassay to check the change of Leptin concentrations in serum and adipose tissue.
RESULTS
Serum Leptin level (10.82+/-0.83) microg/L significantly decreased after an injury of 60-minute ischemia and 30-minute reperfusion versus pre-experimental serum levels (16.46+/-3.21) microg/L; Leptin level in serum was higher than that in adipose tissue (4.466+/-2.63) mg/100g, and they both showed a similar changeable trend to increase step by step as reperfusion time extended (P=0.047).
CONCLUSION
Leptin may be an inflammatory cytokine and may play a role in inflammatory responses such as intestinal ischemia/reperfusion.
目的
探讨肠缺血/再灌注损伤对血清和脂肪组织中瘦素浓度的影响，并明确瘦素在急性炎症反应中的作用。
方法
建立大鼠肠缺血-再灌注损伤模型，采用高灵敏度小鼠瘦素放射免疫分析法检测血清和脂肪组织中瘦素浓度的变化。
结果
60分钟缺血和30分钟再灌注损伤后，血清瘦素水平（10.82±0.83）μg/L较实验前血清水平（16.46±3.21）μg/L显著降低；血清中瘦素水平高于脂肪组织中的（4.466±2.63）mg/100g，且二者均呈现随着再灌注时间延长而逐步升高的相似变化趋势（P = 0.047）。
结论
瘦素可能是一种炎性细胞因子，可能在肠缺血/再灌注等炎症反应中发挥作用。
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