Huang Yong, Zurlinden Elisabeth, Lin Emil, Li Xiaohua, Tokumoto Jason, Golden Jeff, Murr Andrew, Engstrom John, Conte John
Department of Biopharmaceutical Sciences, University of California San Francisco, 94117, San Francisco, CA, USA
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jan 5;799(1):51-61. doi: 10.1016/j.jchromb.2003.10.043.
We have developed a sensitive, high-pressure liquid chromatographic-tandem mass spectrometric (LC/MS/MS) method for the simultaneous determination of didanosine (ddI) and stavudine (d4T) in human plasma, bronchoalveolar lavage fluid (BALF), alveolar cells (AC), peripheral blood mononuclear cells (PBMC), seminal plasma, cerebrospinal fluid (CSF), and tonsil tissue. Plasma, AC, PBMC and CSF were run with an isocratic HPLC method, while BALF supernatant, semen, and tonsil tissue utilized a gradient elution. Samples were prepared by solid phase extraction. Detection was by electrospray positive ionization with multiple reaction monitoring mode. The lower limits of quantitation for both ddI and d4T were 2.0 ng/ml in plasma; 0.5 ng/ml in CSF; 0.4 ng/ml in AC, PBMC, and BALF; 1.0 ng/ml in seminal plasma; and 0.01 ng/mg in tonsil tissue.
我们开发了一种灵敏的高压液相色谱-串联质谱（LC/MS/MS）方法，用于同时测定人血浆、支气管肺泡灌洗液（BALF）、肺泡细胞（AC）、外周血单核细胞（PBMC）、精液、脑脊液（CSF）和扁桃体组织中的去羟肌苷（ddI）和司他夫定（d4T）。血浆、AC、PBMC和CSF采用等度高效液相色谱法进行分析，而BALF上清液、精液和扁桃体组织则采用梯度洗脱。样品通过固相萃取法制备。检测采用电喷雾正离子多反应监测模式。ddI和d4T在血浆中的定量下限均为2.0 ng/ml；在CSF中为0.5 ng/ml；在AC、PBMC和BALF中为0.4 ng/ml；在精液中为1.0 ng/ml；在扁桃体组织中为0.01 ng/mg。
