鲤鱼（Cyprinus carpio）卵黄蛋白原的一步非变性纯化方法。
One-step, non-denaturing purification method of carp (Cyprinus carpio) vitellogenin.
作者信息
Magalhães Ilizabete, Ledrich Marie-Laure, Pihan Jean-Claude, Falla Jaïro
机构信息
Laboratoire d'Immunologie et de Microbiologie (LIM/ESE-CNRS, unité FRE2635), Dept. Genie Biologique, IUT de Thionville-Yutz, 1 Impasse A. Kastler, F-57970 Yutz, France.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jan 5;799(1):87-93. doi: 10.1016/j.jchromb.2003.10.026.
A new single-step purification procedure was developed to purify carp (Cyprinus carpio) vitellogenin (VTG), from estradiol-treated carp plasma. This method was performed by high performance liquid weak anion-exchange chromatography, using a discontinuous elution gradient of NaCl (0-0.5 M, steps of 12.5 mM/4 min). SDS and native-PAGE analysis, of treated-fish plasma and purified solution, showed the appearance of a 370 kDa phospholipoprotein, composed of two 130 kDa monomers, with all VTG characteristics. The sequencing of a 130 kDa monomer confirmed that it was carp VTG. Consequently, this procedure is a rapid method, permitting high quantities of non-denatured carp VTG to be obtained.
开发了一种新的单步纯化程序，用于从经雌二醇处理的鲤鱼血浆中纯化鲤鱼（Cyprinus carpio）卵黄蛋白原（VTG）。该方法通过高效液相弱阴离子交换色谱法进行，使用NaCl的不连续洗脱梯度（0-0.5 M，步长为12.5 mM/4分钟）。对处理过的鱼血浆和纯化溶液进行SDS和天然PAGE分析，结果显示出现了一种370 kDa的磷脂蛋白，由两个130 kDa的单体组成，具有所有VTG的特征。对一个130 kDa单体的测序证实它是鲤鱼VTG。因此，该程序是一种快速方法，能够获得大量未变性的鲤鱼VTG。
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