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Isolation of turbot (Scophthalmus maximus) vitellogenin by high-performance anion-exchange chromatography.

作者信息

Silversand C, Haux C

机构信息

Department of Zoophysiology, University of Göteborg, Sweden.

出版信息

J Chromatogr. 1989 Sep 15;478(2):387-97.

PMID:2600147
Abstract

The use of high-performance anion-exchange chromatography on a Mono Q column for isolation of a glycolipophosphoprotein, vitellogenin, from turbot plasma has been evaluated. The method is an effective, rapid one-step procedure, which gives a pure preparation of vitellogenin as assessed by electrophoresis, [32P]orthophosphate incorporation and amino acid composition.

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