人体结肠中黏膜相关双歧杆菌属、乳酸杆菌属及其他乳酸菌分布的案例研究。
Case study of the distribution of mucosa-associated Bifidobacterium species, Lactobacillus species, and other lactic acid bacteria in the human colon.
作者信息
Nielsen D S, Møller P L, Rosenfeldt V, Paerregaard A, Michaelsen K F, Jakobsen M
机构信息
Department of Dairy and Food Science, Center for Advanced Food Studies, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
出版信息
Appl Environ Microbiol. 2003 Dec;69(12):7545-8. doi: 10.1128/AEM.69.12.7545-7548.2003.
Abstract
The distribution of mucosa-associated bacteria, bifidobacteria and lactobacilli and closely related lactic acid bacteria, in biopsy samples from the ascending, transverse, and descending parts of the colon from four individuals was investigated by denaturing gradient gel electrophoresis (DGGE). Bifidobacterial genus-specific, Lactobacillus group-specific, and universal bacterial primers were used in a nested PCR approach to amplify a fragment of the 16S rRNA gene. DGGE profiles of the bifidobacterial community were relatively simple, with one or two amplicons detected at most sampling sites in the colon. DGGE profiles obtained with Lactobacillus group-specific primers were complex and varied with host and sampling site in the colon. The overall bacterial community varied with host but not sampling site.
摘要
通过变性梯度凝胶电泳（DGGE）研究了来自4名个体的结肠升段、横段和降段活检样本中黏膜相关细菌、双歧杆菌、乳酸杆菌及密切相关乳酸菌的分布情况。采用巢式PCR方法，使用双歧杆菌属特异性引物、乳酸杆菌属特异性引物和通用细菌引物扩增16S rRNA基因片段。双歧杆菌群落的DGGE图谱相对简单，在结肠的大多数采样点最多检测到一两个扩增子。用乳酸杆菌属特异性引物获得的DGGE图谱复杂，且因宿主和结肠采样部位而异。总体细菌群落因宿主而异，但不因采样部位而异。
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