Grande Cristina, Templado José, Cervera J Lucas, Zardoya Rafael
Departamento de Biodiversidad y Biología Evolutiva, Museo Nacional de Ciencias Naturales, CSIC, Madrid, Spain.
Mol Biol Evol. 2004 Feb;21(2):303-13. doi: 10.1093/molbev/msh016. Epub 2003 Dec 5.
A new phylogenetic hypothesis for Euthyneura is proposed based on the analysis of primary sequence data (mitochondrial cox1, trnV, rrnL, trnL(cun), trnA, trnP, nad6, and nad5 genes) and the phylogenetic utility of two rare genomic changes (the relative position of the mitochondrial trnP gene, and an insertion/deletion event in a conserved region of the mitochondrial Cox1 protein) is addressed. Both sources of phylogenetic information clearly rejected the monophyly of pulmonates, a group of gastropods well supported so far by morphological evidence. The marine basommatophoran pulmonate Siphonaria was placed within opisthobranchs and shared with them the insertion of a Glycine in the Cox 1 protein. The marine systellommatophoran pulmonate Onchidella was recovered at the base of the opisthobranch + Siphonaria clade. Opisthobranchs, Siphonaria, and Onchidella shared the relative position of the mitochondrial trnP gene between the mitochondrial trnA and nad6 genes. The land snails and slugs (stylommatophoran pulmonates) were recovered as an early split in the phylogeny of advanced gastropods. The monophyly of the Euthyneura (Opisthobranchia + Pulmonata) was rejected by the inclusion of the heterostrophan Pyramidella.
基于对主要序列数据（线粒体细胞色素氧化酶亚基1、tRNA缬氨酸、16S核糖体RNA、tRNA亮氨酸（UUR）、tRNA丙氨酸、tRNA脯氨酸、烟酰胺腺嘌呤二核苷酸脱氢酶6和烟酰胺腺嘌呤二核苷酸脱氢酶5基因）的分析，提出了一种关于真肺目动物的新系统发育假说，并探讨了两种罕见基因组变化（线粒体tRNA脯氨酸基因的相对位置，以及线粒体细胞色素氧化酶亚基1蛋白保守区域的插入/缺失事件）的系统发育效用。这两种系统发育信息来源均明确否定了肺螺亚纲动物的单系性，肺螺亚纲是一类迄今为止在形态学证据上得到充分支持的腹足纲动物。海洋基眼肺螺亚纲的肺螺类动物虹蛹螺被置于后鳃亚纲动物之中，并且在细胞色素氧化酶亚基1蛋白中与它们共享甘氨酸的插入。海洋全鳃肺螺亚纲的肺螺类动物背孔螺在“后鳃亚纲动物 + 虹蛹螺”分支的基部被发现。后鳃亚纲动物、虹蛹螺和背孔螺共享线粒体tRNA脯氨酸基因在线粒体tRNA丙氨酸基因和烟酰胺腺嘌呤二核苷酸脱氢酶6基因之间的相对位置。陆地蜗牛和蛞蝓（柄眼肺螺亚纲的肺螺类动物）在高级腹足纲动物的系统发育中被发现是早期分化出来的。真肺目动物（后鳃亚纲 + 肺螺亚纲）的单系性因包含异旋目动物金字塔螺而被否定。
