牛胰激肽释放酶：纯化与放射免疫测定

Bovine pancreatic kallikrein: purification and radioimmunoassay.

作者信息

al-Hamidi A A, al-Tufail M A, Bailey G S

机构信息

Department of Chemistry and Biological Chemistry, University of Essex, Colchester, UK.

出版信息

Agents Actions Suppl. 1992;38 ( Pt 1):121-8. doi: 10.1007/978-3-0348-7321-5_16.

DOI:10.1007/978-3-0348-7321-5_16

PMID:1466261

Abstract

Kallikrein was isolated from bovine pancreatic tissue by a sequential procedure of anion-exchange, hydrophobic interaction and gel filtration chromatographies. A monospecific polyclonal antiserum to the pure enzyme was raised in rabbits and was used to set up a specific radioimmunoassay for bovine tissue kallikrein.

摘要

通过阴离子交换、疏水相互作用和凝胶过滤色谱的连续步骤，从牛胰腺组织中分离出激肽释放酶。在兔中制备了针对纯酶的单特异性多克隆抗血清，并用于建立牛组织激肽释放酶的特异性放射免疫测定。