Carroll Ian M, Ahmed Niyaz, Beesley Sarah M, Khan Aleem A, Ghousunnissa Sheikh, Moráin Colm A O, Smyth Cyril J
Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland.
J Clin Microbiol. 2003 Dec;41(12):5755-9. doi: 10.1128/JCM.41.12.5755-5759.2003.
Genotyping of 74 Irish Helicobacter pylori isolates was performed at four different loci (vacA signal sequence and mid-region, insertion-deletion polymorphisms at the 3' end of the cag pathogenicity island, and cagA). The predominant vacA alleles and insertion-deletion motifs suggest an ancestral relationship between Irish isolates and either specific East Asian or Northern European strains. In addition, fluorescent amplified fragment length polymorphism-PCR genotyping and phylogenetic analysis of 32 representative Irish H. pylori isolates and 22 isolates from four different continents demonstrated that the Irish H. pylori isolates examined were weakly clonal and showed some association with both European and Asian isolates. These three genotyping techniques show that Irish H. pylori isolates have distinctive features that may have evolved in this insular European population.
对74株爱尔兰幽门螺杆菌分离株在四个不同位点进行基因分型（vacA信号序列和中间区域、cag致病岛3'端的插入缺失多态性以及cagA）。主要的vacA等位基因和插入缺失基序表明爱尔兰分离株与特定的东亚或北欧菌株之间存在祖先关系。此外，对32株具有代表性的爱尔兰幽门螺杆菌分离株和来自四大洲的22株分离株进行荧光扩增片段长度多态性-PCR基因分型和系统发育分析表明，所检测的爱尔兰幽门螺杆菌分离株克隆性较弱，并且与欧洲和亚洲分离株均有一定关联。这三种基因分型技术表明，爱尔兰幽门螺杆菌分离株具有独特特征，这些特征可能是在这个欧洲岛屿人群中进化而来的。
