评估基于聚合酶链反应的通用异源双链体生成检测法作为秘鲁快速检测耐多药结核分枝杆菌工具的效果。
Evaluation of a PCR-based universal heteroduplex generator assay as a tool for rapid detection of multidrug-resistant Mycobacterium tuberculosis in Peru.
作者信息
Mayta Holger, Gilman Robert H, Arenas Fanny, Valencia Teresa, Caviedes Luz, Montenegro Sonia H, Ticona Eduardo, Ortiz Jaime, Chumpitaz Rosa, Evans Carlton A, Williams Diana L
机构信息
Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA.
出版信息
J Clin Microbiol. 2003 Dec;41(12):5774-7. doi: 10.1128/JCM.41.12.5774-5777.2003.
Abstract
Multidrug-resistant tuberculosis is an increasing health problem worldwide, especially in developing countries. The PCR-UHG-Rif assay, which detects mutations within the rpoB gene associated with rifampin resistance, was evaluated for its ability and reliability to detect and identify drug-resistant Mycobacterium tuberculosis in a developing country where tuberculosis is highly endemic.
摘要
耐多药结核病在全球范围内正成为一个日益严重的健康问题，在发展中国家尤为如此。PCR-UHG-Rif检测法可检测与利福平耐药相关的rpoB基因内的突变，该检测法在结核病高度流行的一个发展中国家，就其检测和鉴定耐药结核分枝杆菌的能力及可靠性进行了评估。
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Evaluation of a PCR-based universal heteroduplex generator assay as a tool for rapid detection of multidrug-resistant Mycobacterium tuberculosis in Peru.评估基于聚合酶链反应的通用异源双链体生成检测法作为秘鲁快速检测耐多药结核分枝杆菌工具的效果。
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