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真菌性足菌肿病原体的分子检测与鉴定:两例详细报告

Molecular detection and identification of agents of eumycetoma: detailed report of two cases.

作者信息

Ahmed Abdalla O A, Desplaces Nicole, Leonard Philippe, Goldstein Fred, De Hoog Sybren, Verbrugh Henri, van Belkum Alex

机构信息

Mycetoma Research Group, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.

出版信息

J Clin Microbiol. 2003 Dec;41(12):5813-6. doi: 10.1128/JCM.41.12.5813-5816.2003.

Abstract

We describe two cases of eumycetoma in the legs. The infections could not be adequately diagnosed by classical mycology, but the causative agents were successfully identified as Madurella mycetomatis by species-specific PCR and DNA sequencing.

摘要

我们描述了两例腿部足菌肿病例。传统真菌学方法无法对这些感染进行充分诊断,但通过种特异性聚合酶链反应(PCR)和DNA测序,成功鉴定出病原体为马杜拉足肿菌。

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本文引用的文献

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Mycetoma.足菌肿
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Mycetoma of the foot caused by Cylindrocarpon destructans.由毁灭柱孢菌引起的足部足菌肿
J Clin Microbiol. 1991 Sep;29(9):1855-9. doi: 10.1128/jcm.29.9.1855-1859.1991.
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Rapid and simple method for purification of nucleic acids.快速简便的核酸纯化方法。
J Clin Microbiol. 1990 Mar;28(3):495-503. doi: 10.1128/jcm.28.3.495-503.1990.

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