Won Lisa, Bubula Nancy, Hessefort Suzanne, Gross Martin, Heller Alfred
Department of Neurobiology, Pharmacology and Physiology, The University of Chicago, 947 East 58th Street, Chicago, IL 60637, USA.
Neurosci Lett. 2003 Dec 19;353(2):83-6. doi: 10.1016/j.neulet.2003.08.081.
Lysates of X61, a striatal-derived cell line, and a partially purified preparation from the lysate (UF4) contain a factor(s) capable of increasing the dopamine content of a mesencephalic-derived dopaminergic cell line (MN9D) and of cultures containing primary dopaminergic neurons. Treatment of cultures containing dopaminergic primary neurons grown in the absence of target cells over a 2 week period with X61 lysate or UF4 resulted in an elevation of dopamine levels of the cultures and of media homovanillic acid as well as a 2.0-fold (UF4) to 2.9-fold (X61 lysate) increase in the density of dopaminergic neurons in treated cultures. The results suggest that the activity factor derived from X61 is capable of preventing dopaminergic cell loss which occurs in the absence of dopaminergic target cells of the corpus striatum.
源自纹状体的细胞系X61的裂解物，以及该裂解物的部分纯化制剂（UF4）含有一种因子，能够增加源自中脑的多巴胺能细胞系（MN9D）以及含有原代多巴胺能神经元的培养物中的多巴胺含量。用X61裂解物或UF4处理在无靶细胞的情况下培养2周的含有多巴胺能原代神经元的培养物，导致培养物和培养基高香草酸中的多巴胺水平升高，并且处理后的培养物中多巴胺能神经元密度增加了2.0倍（UF4）至2.9倍（X61裂解物）。结果表明，源自X61的活性因子能够防止在缺乏纹状体多巴胺能靶细胞的情况下发生的多巴胺能细胞损失。
