Barr S C, Bowman D D, Erb H N
Department of Clinical Sciences, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Am J Vet Res. 1992 Nov;53(11):2028-31.
Two commercially available tests, an antigen-capture ELISA for use on fecal samples, and a peroral nylon string test for use in dogs, were compared with a zinc sulfate fecal concentration technique (ZSCT) for detection of giardiasis in dogs. Of 77 dogs and 164 fecal samples (from these dogs), 33 and 52, respectively were found to be Giardia-positive on the basis of results of the ZSCT. The ELISA gave false-negative results for 10 and 14% of ZSCT-positive dogs and fecal samples, respectively, and false-positive results (relative to the ZSCT test results) in 13 and 10% of ZSCT-positive dogs and fecal samples, respectively. Of the 18 string-tested dogs, 14 were positive by results of the ZSCT. Of the 4 dogs that were Giardia-negative by ZSCT, 2 were Giardia-positive by ELISA. Dogs were sedated and given water and metoclopramide to aid passage into the duodenum of the capsule containing a nylon string. Of the 21 string tests performed on the 18 dogs, only 5 strings reached the duodenum, and 0 of the 5 yielded positive results for Giardia sp. Because the string broke in 1 dog (leaving most in the gastrointestinal tract and, therefore, producing a risk of string foreign body) further string tests were not done.
将两种市售检测方法与硫酸锌粪便浓缩技术（ZSCT）进行比较，以检测犬贾第虫病。这两种方法分别是用于粪便样本的抗原捕获酶联免疫吸附测定（ELISA）和用于犬的口服尼龙绳检测法。在77只犬和164份粪便样本（来自这些犬）中，根据ZSCT结果，分别有33只犬和52份粪便样本被发现贾第虫呈阳性。ELISA分别对10%和14%的ZSCT阳性犬和粪便样本给出假阴性结果，对13%和10%的ZSCT阳性犬和粪便样本给出假阳性结果（相对于ZSCT检测结果）。在18只接受绳检的犬中，14只根据ZSCT结果呈阳性。在4只经ZSCT检测贾第虫呈阴性的犬中，2只经ELISA检测贾第虫呈阳性。给犬镇静，然后给予水和甲氧氯普胺，以帮助装有尼龙绳的胶囊进入十二指肠。在对18只犬进行的21次绳检中，只有5根绳子到达十二指肠，这5根绳子中没有一根对贾第虫属检测呈阳性结果。由于有1只犬的绳子断裂（大部分留在胃肠道内，因此有产生绳状异物的风险），所以没有进一步进行绳检。
