来自波罗的海格但斯克湾的波罗的海蛤（双壳纲：海螂科）正常和肿瘤鳃细胞间期和中期的嗜银核仁组织区（AgNORs）

Argyrophilic nucleolar organizer regions (AgNORs) in interphases and metaphases of normal and neoplastic gill cells of Macoma balthica (Bivalvia: Tellinidae) from the Gulf of Gdansk, Baltic Sea.

作者信息

Smolarz K, Wolowicz M, Thiriot-Quiévreux C

机构信息

Laboratory of Estuarine Ecology, Institute of Oceanography, University of Gdansk, Al. Marszalka, J. Pilsudskiego, 81-378 Gdynia, Poland.

出版信息

Dis Aquat Organ. 2003 Oct 24;56(3):269-74. doi: 10.3354/dao056269.

DOI:10.3354/dao056269

PMID:14667039

Abstract

Chromosome analysis of gill cells of different populations of Macoma balthica (L.) from the Bay of Gdansk (Baltic Sea) revealed 2 clam categories, 1 with neoplastic features and 1 without. Silver-staining was performed on interphase and metaphase cells of both categories. The mean argyrophilic nucleolar organizer region (AgNOR) count per abnormal interphase cell was significantly higher than in normal interphase cells. Normal silver-stained metaphases had 3 nucleolar organizer region (NOR) chromosome phenotypes. The location of the NORs in the most frequent phenotype (55.6% in 54 metaphases scored) was interstitial on the largest metacentric chromosome pair, Pair No. 1. Abnormal silver-stained metaphases had a higher number of active NOR sites. Different phenotypes were observed (frequency greater than 10% for 67 metaphases scored); 2 were similar to those in normal metaphases and 5 were ectopic. The higher activity of AgNORs observed in abnormal cells confirmed the diagnosis of malignant neoplasia.

摘要

对来自格但斯克湾（波罗的海）的不同波罗的海蛤仔种群鳃细胞进行染色体分析，发现了2种蛤类，1种具有肿瘤特征，1种没有。对这两类细胞的间期和中期细胞进行了银染。每个异常间期细胞的平均嗜银核仁组织区（AgNOR）计数显著高于正常间期细胞。正常银染中期有3种核仁组织区（NOR）染色体表型。在最常见的表型中（在54个计分的中期细胞中占55.6%），NOR位于最大的中着丝粒染色体对（第1对）的中间位置。异常银染中期有更多的活性NOR位点。观察到不同的表型（在67个计分的中期细胞中频率大于10%）；2种与正常中期相似，5种是异位的。在异常细胞中观察到的AgNOR活性较高，证实了恶性肿瘤的诊断。